Activation and Transportation of Fatty Acids to the Mitochondria via the Carnitine Shuttle with Animation Animations PharmaXChange.info Function of carnitine in the transport of mitochondrial long chain Download Scientific Diagram Short Note On Carnitine Shuttle: Fatty acids are oxidized in mitochondria, and synthesized in the cytoplasm. Long chain fatty acids must form an active intermediate, fatty acyl CoA, before being oxidized inside mitochondria. Catherine Critical Role of Mitochondrial Fatty Acid Metabolism in Normal Cell Function and Pathological Conditions