Adaptation to acetaminophen exposure elicits major changes in expression and distribution of the hepatic proteome Scientific Reports Acetaminophen and aspirin toxicity are both high yield, but the injury pattern is very different. Acetaminophen overdose overwhelms glutathione, allowing NAPQI to build up and cause hepatic necrosis. The antidote is N acetylcysteine, which File:Acetaminophen metabolism.jpg Wikimedia Commons Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.