The molecular mechanisms of acetaminophen induced hepatotoxicity and its potential therapeutic targets Guangwen Luo, Lili Huang, Zhaowei Zhang, 2023 Sex differences in glutathione metabolism and acetaminophen toxicity Circulating acetaminophen metabolites are toxicokinetic biomarkers of acute liver injury Vliegenthart 2017 Clinical Pharmacology & Therapeutics Wiley Online Library Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.