Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. How Keeping Glutathione Levels Up Can Benefit Your Health & Your Vitamin D Status GrassrootsHealth What is Glutathione and How Do I Get More of It? Mark Hyman, MD The Glutathione and Vitamin D Relationship GrassrootsHealth