Silymarin prevents acetaminophen induced hepatotoxicity via up regulation of the glutathione conjugation capacity in mice ScienceDirect Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Acetaminophen Toxicity and Liver Zone Physiology [#DaVinciCases GI 5 Physiology Case 3] Is Tylenol Bad for Your Liver or Kidneys? GoodRx