Acetaminophen Toxicity: How Genetic Susceptibility Combines with Environmental Factors Illustration of the effects of acetaminophen on glutathione, Download Scientific Diagram Acetaminophen Pathway (therapeutic doses), Pharmacokinetics Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.