Metabolism of acetaminophen (paracetamol) in plantstwo independent pathways result in the formation of a glutathione and a glucose conjugate Environmental Science and Pollution Research Springer Nature Link Clinical toxicity of Acetaminophen Frontiers Herbal Therapy for the Treatment of Acetaminophen Associated Liver Injury: Recent Advances and Future Perspectives Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.