Silymarin prevents acetaminophen induced hepatotoxicity via up regulation of the glutathione conjugation capacity in mice ScienceDirect The Metabolism of Acetaminophen and the Synthesis of Glutathione Download Scientific Diagram Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Tylenol's Impact on Glutathione and Liver Damage