Tylenol: How much is too much of a good thing? Allied Medical Training The biochemistry of acetaminophen hepatotoxicity and rescue: a mathematical model Theoretical Biology and Medical Modelling Springer Nature Link Can Tylenol (acetaminophen) deplete glutathione levels in the body? Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors.