Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. Homocysteine May Decrease Glucose Uptake and Alter the Akt GSK3 GLUT1 Signaling Pathway in Hippocampal Slices: Neuroprotective Effects of Rivastigmine and Ibuprofen Molecular Neurobiology Springer Nature Link Tylenol's Impact on Glutathione and Liver Damage Which analgesic causes liver toxicity?