Could Tylenol be making your child's health worse? Acetaminophen is widely used for pain and fever relief, but research suggests it depletes glutathione, a powerful antioxidant essential for immune function, Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione Depletion: Cell Metabolism Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. The gamma glutamyl pathway after glutathione depletion due to Download Scientific Diagram